IHC analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1). IL-2 Receptor alpha/Il2ra was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-2 Receptor alpha/Il2ra Antibody (A00214-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Western blot analysis of IL-2 Receptor alpha/Il2ra using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse ANA-1 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates, Lane 3: mouse RAW264.7 whole cell lysates, Lane 4: mouse HEPA1-6 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat RH35 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-2 Receptor alpha/Il2ra antigen affinity purified polyclonal antibody (Catalog # A00214-1) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-2 Receptor alpha/Il2ra at approximately 50 kDa. The expected band size for IL-2 Receptor alpha/Il2ra is at 50 kDa.

Flow Cytometry analysis of HEPA1-6 cells using anti-IL-2 Receptor alpha/Il2ra antibody (A00214-1). Overlay histogram showing HEPA1-6 cells stained with A00214-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL-2 Receptor alpha/Il2ra Antibody (A00214-1, 1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.